胎牛血清外泌体去除试剂盒 FBS Exosome Depletion Kit Exosome Depletion-北京博迈斯科技发展有限公司-Norgen biotek-「北京博迈斯科技发展有限公司

胎牛血清外泌体去除试剂盒 Exosome Depletion-北京博迈斯科技发展有限公司

FBS Exosome Depletion Kit (Slurry Format)

产品概述：

Efficient depletion of cow's exosomes from Fetal Bovine Serum

Deplete exosome-sized vesicles from versatile FBS volumes of up to 280 mL

No protease treatment required

No time-consuming ultracentrifugation

No precipitation reagents required

No overnight incubation required

Depleted FBS has no detectable cow’s miRNA

The depleted FBS provides the same cellular growth rates as the standard FBS

试剂盒成分组成：

试剂盒名称/货号试剂盒成分	FBS Exosome Depletion Kit I(Slurry Format) Cat# 61100 Up to 140 mL	FBS Exosome Depletion Kit II(Slurry Format) Cat# 61400 Up to 280 mL
Number of Preps	6 preps	12 preps
ExoC Buffer	2 x 1.5 mL	8 mL
Slurry E	12.5 mL	2 x 12.5 mL
Product Insert	1	1

Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature (15-25ºC) for up to 2 years without showing any reduction in performance.

Customer-Supplied Reagents and Equipment • Disposable powder-free gloves • Swinging bucket centrifuges. • Vortexer • Sterile pipette tips with filters • 50 mL conical tubes • 0.2µ Filter • DMEM, RPMI or other base media

操作步骤：

Section 1: FBS Exosome Depletion

Note: The procedure outlined below is for 20 mL inputs of FBS. If processing a sample volume lower than 20 mL media, simply add 25 µL ExoC Buffer, 100 µL Slurry E and 250 µL from your media of interest for every 1mL of FBS.

1. To 20 mL FBS add 5 mL media of your interest followed by the addition 400 µL of ExoC Buffer and 2 mL of Slurry E (Note: Mix Slurry E well prior to use. For optimal performance ensure that resin is completely resuspended).

2. Mix well by vortexing for 10 seconds and let stand at room temperature for 10 minutes.

3. Mix well by vortexing for 10 seconds. Centrifuge for 2 minutes at 2,000 RPM. Do Not Discard the supernatant. (The supernatant from step 3 contains your Exosome-depleted FBS).

4. Transfer the supernatant into a fresh 50 cc tube (not provided) without disturbing the slurry pellet.

5. Filter the supernatant using a 0.2 µM Filter (not provided).

6. Aliquot your Exosome-depleted FBS filtrate and store it at -20oC for future use

➢ Your FBS is now Exosome-depleted and ready to be used as a as a growth supplement in your culture medium.

Section 2: Media Preparation with Exosome-Depleted FBS

1. Thaw the Exosome-Depleted FBS for overnight at 4oC.

2. Combine 63.5 mL of your Exosome-Depleted FBS and 5 mL of your Antibiotic - Antimycotic stock of interest in 500 mL media of interest (Note: your media of interest should be the same media used to dilute your FBS during the depletion process in Section 1, Step 1)