K4901-100? Antibody coated 96-well plate (12 x 8-well strips) ? 10X Wash concentrate, 50 ml ? 5X Diluent, 50 ml ? Secondary antibody, 12 ml ? 100X Detector, 150 μl ? Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized ? QC sample= positive control having 7-11 μg/ml human adiponectin, 1 vial, lyophilized ? Substrate I, 6 ml ? Substrate II, 6 ml ? Stop solution, 12 mlCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluidsHuman Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in Human Serum, Plasma or Various Tissue or Cell Culture supernatants. 100 Assays.Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.