K4802-100? Angiogenin Microplate (Item A) coated with anti-human monoclonal Ab against Angiogenin, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Angiogenin) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. 5x concentrated ? Detection Antibody Angiogenin (Item F), biotinylated anti-human Angiogenin (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 700x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineAngiogenin (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Angiogenin in serum, plasma, urine & cell culture supernatants. Detection Range: 1.5 pg/ml - 400 pg/ml. 100 assays. BioVision’s Human Angiogenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiogenin. This assay employs an antibody specific for human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of Angiogenin is typically less than 1.5 pg/ml. Detection Range: 1.5 pg/ml – 400 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.