K4916-100? 1 plate coated with human ANGPTL6 Antibody ? Wash Buffer 10X ? Diluent 5X ? Detection Antibody ? Detector 100X (HRP Conjugated anti-rabbit IgG) ? human ANGPTL6 Standard (lyophilized) ? human ANGPTL6 QC sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL6 (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ANGPTL6 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 1.56 – 100 ng/ml and Detection limit 1.2 ng/ml. 100 Assays. Seven angiopoietin-like proteins (ANGPTLs) share the characteristic protein structure of the angiopoietin family (ANG), but differ in their inability to bind antiopoietin receptor, Tie-2. ANGPTL6 was originally named angiopoietin-related growth factor (AGF) having an N-terminal coiled-coil-like domain and a C-terminal fibrinogen-like domain, both of which are conserved in ANG. It is a circulating protein secreted by liver that induces angiogenesis by direct effect of epidermal ANGPTL6 on endothelial cells and proliferation of skin cells, and thereby promotes wound healing. 80% of Angptl6 -/- mice died at about embryonic day 13. The surviving null mice developed marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure relative to controls. Conversely, mice with constitutive overexpression of ANGPTL6 showed leanness and increased insulin sensitivity resulting from increased energy expenditure, and were also protected from high-fat diet-induced obesity, insulin resistance, and non-adipose tissue steatosis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL6 in biological fluids. A monoclonal antibody specific for ANGPTL6 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL6 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL6 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL6 in the samples. The assay range is 1.56 – 100 ng/ml ANGPTL6/ml. The lowest level of ANGPTL6 that can be detected by this assay is 1.2 ng/ml.