K4803-100? CA-125 Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CA-125 Standard (Item C) ? Assay Diluent (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (800x ) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCancer Antigen 125 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA-125 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.6 U/ml - 400 U/ml. 100 assays. BioVision’s Human Cancer Antigen 125 (CA-125) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-125. This assay employs an antibody specific for human CA-125 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA-125 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA-125 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA-125 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA-125 is typically less than 0.6 U/ml. Detection Range: 0.6 U/ml – 400 U/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.