K3073-100? CD40 Microplate (Item A) coated with anti-mouse CD40, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse CD40) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. 5x concentrated ? Detection Antibody CD40 (Item F), biotinylated anti-mouse CD40 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants CD40 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse CD40 in serum, plasma & cell culture supernatants. Detection Range: 3 pg/ml - 1500 pg/ml. 100 assays. BioVision’s mouse CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40. This assay employs an antibody specific for mouse CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of CD40 is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 1500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.