K4387-100? Microplate coated with anti-human SDF-1 alpha ? Wash Buffer (20x) (Item B) ? Human SDF-1 alpha Standard (Item C) ? Assay Diluent A (Item D) (0.09% NaN3) ? Assay Diluent B (Item E) (5x concentrated) ? Detection Antibody SDF-1 alpha (Item F) ? HRP-Streptavidin (Item G), (200x) ? TMB (Item H) ? Stop Solution (Item I) ? Plasma (human SDF-1 alpha conc. is pretty low in normal plasma, it may not be detected. serum samples are not recommended) ? Cell culture supernatants ? Urine CXCL12/SDF-1 alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CXCL12/SDF-1 alpha in plasma, cell culture supernatants & urine. Detection Range: 80 pg/ml – 6,000 pg/ml. 100 assays.BioVision’s Human SDF-1 (Stromal cell-derived factor-1) alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF-1 alpha. This assay employs an antibody specific for human SDF-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SDF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 80 pg/ml. Detection Range: 80 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.