K4801-100? CD26 Microplate (Item A) coated with anti-human CD26, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD26) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative. ? Assay Diluent B (Item E) for Standard/Sample (cell culture supernatant/urine) (5x concentrated) ? Detection Antibody CD26 (Item F), biotinylated anti-human CD26 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & Plasma ? Cell culture supernatants ? urine DPP IV/CD26 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD26 in serum, plasma, urine & cell culture supernatants. Detection Range: 25 pg/ml - 8,000 pg/ml. 100 assays. BioVision’s human DPP IV (Dipeptidyl peptidase-4) or CD26 (cluster of differentiation 26) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD26. This assay employs an antibody specific for CD26 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD26 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD26 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD26 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of CD26 is typically less than 25 pg/ml. Detection Range: 25 pg/ml – 8,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.