K4022-100? EGF Microplate coated with anti-human monoclonal Ab against EGF, 96 wells ? Lyophilized recombinant human EGF standard (10 ng/vial) ? Biotinylated anti- human EGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (heparin, EDTA, citrate) ? Cell culture supernatants ? Urine EGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human EGF in serum, plasma, urine & cell culture supernatants. Detection Range: 4.7-300 pg/ml. 100 assays.BioVision’s human EGF (Epidermal Growth Factor) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for EGF coated on a 96-well plate. Standards (E. Coli, N971-R1023) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human EGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 4.7 pg/ml – 300 pg/ml. Sensitivity: < 1 pg/ml.