K4773-100? IFN-γ Microplate (Item A) coated with anti-human IFN-γ, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IFN-γ) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody IFN-γ (Item F), biotinylated anti-human IFN-γ (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human IFN-γ concentration is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine IFN-gamma (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IFN-gamma in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 15 pg/ml - 15000 pg/ml. 100 assays. BioVision’s Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ. This assay employs an antibody specific for human IFN-γ coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-γ present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-γ antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IFN-γ is typically less than 15 pg/ml. Detection Range: 15 pg/ml - 15000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.