K4775-100? IGF-I Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human IGF-I Standard (Item C) ? Assay Diluent C (Item L) ? Detection Antibody (Item F) ? HRP-Streptavidin (120x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? Urine IGF-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IGF-1 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.1 ng/ml - 30 ng/ml. 100 assays. BioVision’s human IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I. This assay employs an antibody specific for human IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit only can detect a free form of human IGF-I & shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IGF-I is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml - 30 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.