K4786-100? MIF Microplate (Item A) coated with anti-human MIF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human MIF) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody MIF (Item F), biotinylated anti-human MIF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 300x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (collect plasma using heparin as an anticoagulant. EDTA & citrate are not recommended. ? Cell culture supernatants ? Urine MIF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human MIF in serum, plasma, urine & cell culture supernatants. Detection Range: 6 pg/ml – 6,000 pg/ml. 100 assays. BioVision’s Human MIF (Migration Inhibitor Factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIF. This assay employs an antibody specific for human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of MIF is typically less than 6 pg/ml. Detection Range: 6 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.