K4785-100? MIP-1 β antibody-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? rh MIP-1 β Standards (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? MIP-1 β Detection Antibody (Item F) ? HRP-Streptavidin (600x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? Urine MIP-1 Beta (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human MIP-1 beta in serum, plasma, urine & cell culture supernatants. Detection Range: 2.5 pg/ml – 1,000 pg/ml. 100 assays. BioVision’s Human MIP-1 beta (MIP-1 β) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 β. This assay employs an antibody specific for human MIP-1 β coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 β present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 β antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 β bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF). The minimum detectable dose of MIP-1 β is typically less than 2.5 pg/ml. Detection Range: 2.5 pg/ml – 1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.