K4762-100? Mouse periostin Standard (Lyophilized) (5 μg) (STD) ? Detection Antibody (20 μl) (DET) ? HRP Labeled Streptavidin (lyophilized) (2 μg) (STREP-HRP) ? 10X Wash Buffer (2 X 30 ml) ? 10X ELISA Buffer (2 X 30 ml) ? TMB Substrate Solution (12 ml) (TMB) ? Stop Solution (12 ml) (STOP) ? Plate coated with periostin Antibody (6 X 16-well strips) ? Plate Covers (plastic film) ? Silica Gel Minibags? Serum & plasma ? Cell culture supernatants Periostin/OSF-2 (mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse Periostin in Serum, Plasma & Cell culture supernatants. Detection Limit: 10 pg/ml. 100 assays. BioVision’s Periostin (mouse) ELISA kit is to be used for the in vitro quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples. Detection limit: 10 pg/ml. Note: The Limit of detection was measured by adding three standard deviations to the mean value of 50 zero standard. Assay Range: 31.25 pg/ml – 2000 pg/ml. Linearity: Different samples containing mouse periostin were diluted several fold (1/4000 to 1/8000) and the measured recoveries ranged from 95% to 105%. Mouse periostin levels range in plasma or serum from 1 to >10 μg /ml. Recovery: When samples (serum or plasma) are spiked with known concentrations of mouse periostin, the recovery averages 96% (range from 89% to 115%).