K4771-100? RANTES Microplate (Item A) coated with anti-mouse RANTES, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse RANTES) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent C (Item L) for Standard/Sample (cell culture medium) diluent ? Assay Diluent B (Item E) for Detection Antibody & HRP-Streptavidin diluent. (5x concentrated) ? Detection Antibody RANTES (Item F), biotinylated anti-mouse RANTES (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants RANTES (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse RANTES in Serum, Plasma & Cell culture supernatants. Detection Range: 1 pg/ml - 200 pg/ml. 100 assays. BioVision’s Mouse RANTES (Regulated upon activation, Normal T cell Expressed and presumably Secreted) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RANTES. This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of RANTES is typically less than 1 pg/ml. Detection Range: 1 pg/ml - 200 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.