K4750-100? Serum & plasma ? Cell culture supernatants TIMP-1 (Mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse TIMP-1 in Serum, Plasma & Cell culture supernatants. Detection Limit: less than 3 pg/ml. 100 assays.BioVision’s Mouse TIMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP-1. This assay employs an antibody specific for mouse TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TIMP-1 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.