K5363-100? Plate coated with anti-human VEGF antibody, 96-wells ? Human VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-human VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatants ? UrineVEGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 31.2 pg/ml – 2000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. VEGF mediates progression of Diabetic retinopathy as well as cancer metastasis. BioVision’s human VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 31.2 pg/ml – 2000 pg/ml. Sensitivity: < 1 pg/ml.