K4918-100? 1 plate coated with human ACE2 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial human ACE2 Standard (lyophilized) ? 1 vial human ACE2 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsACE2 (human) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ACE2 in Urine, Cell Culture Supernatant etc.. Assay Range 0.391–25 ng/ml and Detection limit 293 pg/ml.100 Assays. By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme, a full-length ACE2 cDNA, originally called ACEH, was isolated, which encoded a deduced 805-amino acid protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. Northern blot analysis detected high expression of ACE2 in kidney, testis, and heart, and moderate expression in colon, small intestine, and ovary. The soluble, truncated form of ACE2 lacks the transmembrane and cytosolic domains in CHO cells and is glycosylated protein that was able to cleave angiotensin I and angiotensin II , but not bradykinin. ACE converts angiotensin I to angiotensin II, which has 8 amino acids, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. This can then further be converted by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Using ACE2 null mice, Crackower et al. showed that ACE2 was critically involved in a cardiac contractility. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ACE2 in biological fluids. A polyclonal antibody specific for ACE2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ACE2 is recognized by the addition of a biotinylated polyclonal antibody specific for ACE2 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ACE2 in the samples. The assay range is 0.391–25 ng/ml ACE2/ml. The lowest level of ACE2 that can be detected by this assay is 293 pg/ml.