K7570-100? 96-well plate coated with anti-CYP3A4 Ab ? Human CYP3A4 standard (20 ng/vial) ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X concentrate) ? Assay diluent A (2X concentrate) ? Assay diluent B (2X concentrate) ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from human tissues or cells Cytochrome P450 3A4 (CYP3A4) human ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human CYP3A4 in tissues, cells or microsomes. Sensitivity: 0.128 ng/ml. Detection Range: 0.128 ng/ml – 20 ng/ml. 100 assays. Cytochrome P450 3A4 (CYP3A4) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in liver and intestinal cells. CYP3A4 alone metabolizes ~50% of all drugs on the market. BioVision’s human CYP3A4 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal human CYP3A4-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to CYP3A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain CYP3A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the human CYP3A4 captured onto the plate. The concentration of CYP3A4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.128 ng/ml – 20 ng/ml. Sensitivity: 0.128 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%.