K926-100? βIG-H3 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials of recombinant human βIG-H3. ? Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody βIG-H3 (Item F): 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl 300x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. Serum, Plasma, Cell culture supernatants and urineHuman βIG-H3 ELISA Kit: Measure human βIG-H3 in Serum, Plasma, Cell culture Supernatants, Urine etc. Direct, Convenient Colorimetric Method. 100 assays.BioVision’s Human βIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human βIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human βIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and βIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human βIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of βIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.