biovision K109-400 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-Biotin Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.