biovision K103-25 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 649 nm; Em = 670 nm) and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) Annexin V-Cy5 Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis within 10 min. Detection: Flow Cytometry or Fluorescence Microscopy Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.