biovision K165-100 ? Cell and tissue lysates ? Purified Cathepsin E ? Detection method- Fluorescence (Ex/Em = 320/420 nm) ? Applications- Detection of Cathepsin E activity in tissue and cell lysates - Determine activity of purified Cathepsin E Cathepsin E Activity Assay Kit: Rapid & Convenient Fluorometric Assay to measure Cathepsin E Activity. Detection limit: 1 mU. 100 Assays. Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. BioVision’s Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.