MAD2 Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot, Immunoprecipitation Western blotting (1:500 – 1:2000) and Immunoprecipitation. HeLa cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MAD2 of human origin. Reactivity to other species has not been tested. Reactivity with mouse samples is likely due to high sequence homology.