K4793-100? Mouse IL-1 alpha Ab coated plate (Item A), 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Mouse IL-1 alpha Standard (Item C) ? Assay Diluent D (5x) (Item K) ? Assay Diluent B (5x) (Item E) ? Biotinylated anti-mouse IL-1 alpha Ab (Item F) ? HRP-Streptavidin Concentrate (500x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants IL-1α (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse IL-1 alpha in serum, plasma & cell culture supernatants. Detection Range: 10 pg/ml - 3000 pg/ml. 100 assays. BioVision’s mouse IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 alpha. This assay employs an antibody specific for human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF). The minimum detectable dose of IL-1 alpha is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.