K7116-100? Angiopoietin-2 Microplate (Item A) coated with anti-human Angiopoietin-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Angiopoietin-2) ? Assay Diluent (Item E) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent (5X) ? Detection Antibody Angiopoietin-2 (Item F), biotinylated anti-human Angiopoietin-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineAngiopoietin-2 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human ANGPT2 in serum, plasma, urine & cell culture supernatants. Detection Range: 10 pg/ml - 3000 pg/ml. 100 assays. BioVision’s Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2. This assay employs an antibody specific for human Angiopoietin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, Angiopoietin-1, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13,IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Angiopoietin-2 is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.