K4747-100? BMP-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-5. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-5. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-5 (Item F): 2 vial of biotinylated anti human BMP-5 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 300x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma (BMP-5 concentration in human normal serum/plasma is pretty low, it may not be detected in this assay) ? Cell culture supernatants ? Urine BMP-5 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-5 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 0.25 ng/ml. 100 assays.BioVision’s Human BMP-5 (Bone morphogenetic proteins-5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-5. This assay employs an antibody specific for human BMP-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-5 is typically less than 0.25 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.