K4805-100? CEA Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CEA Standard (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (1000x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCarcinoembryonic Antigen (CEA) (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CEA in serum, plasma, urine & cell culture supernatants. Detection Range: 0.2 ng/ml - 250g/ml. 100 assays. BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CEA is typically less than 200 pg/ml. Detection Range: 0.2 ng/ml – 250 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.