K3072-100? CD40 Microplate (Item A) coated with anti-human CD40, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD40) ? Assay Diluent D (Item K) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent. 5x concentrated ? Assay Diluent B (Item E) for detection antibody & HRP-Streptavidin diluent. 5x concentrated ? Detection Antibody CD40 (Item F), biotinylated anti-human CD40 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineCD40 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD40 in serum, plasma, urine & cell culture supernatants. Detection Range: 50 pg/ml - 40000 pg/ml. 100 assays. BioVision’s Human CD40 (TNFRSF5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CD40 is typically less than 50 pg/ml. Detection Range: 50 pg/ml – 40000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.