K4770-100? DPP IV Ab coated Microplate (Item A), 96 wells ? Wash Concentrate (20x) (Item B) ? Mouse DPP IV Standards (Item C) ? Assay Diluent C (Item L) ? Assay Diluent B (5x) (Item E) ? Biotinylated anti-mouse DPP IV Ab (Item F) ? HRP-Streptavidin (200x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & Plasma ? Cell culture supernatants DPP4 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse DPP 4 in Serum, Plasma & Cell culture supernatants. Detection Range: 0.45 ng/ml - 100 ng/ml. 100 assays. BioVision’s Mouse DPP IV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DPP IV. This assay employs an antibody specific for DPP IV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP IV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPP IV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP IV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of DPP IV is typically less than 0.45 ng/ml. Detection Range: 0.45 ng/ml - 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.