K4782-100? HGF Microplate (Item A) coated with anti-human HGF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human HGF) ? Sample Diluent Buffer (Item D) for Standard/Sample (cell lysate/tissue lysate) diluent (5x concentrated) ? Assay Diluent (Item E) for Detection Antibody & HRP-Streptavidin concentrate diluent (5x concentrated) ? Detection Antibody HGF (Item F), biotinylated anti-human HGF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Cell Lysate buffer (Item J) (2x concentrated) ? Cell lysates ? Tissue lysates HGF (human) ELISA Kit (For Lysates): Colorimetric Assay for Quantitative measurement of human HGF in Cell & tissue lysates. Detection Limit: less than 3 pg/ml. 100 assays. BioVision’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of HGF is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF).