K4739-100? IL-15 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human IL-15. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials of recombinant human IL-15. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody IL-15 (Item F): 2 vial of biotinylated anti-human IL-15 (each vial is enough to assay half microplate). ? HRP-Streptavidin concentrate (Item G): 200 μl of 200x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’- tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum, plasma (collect plasma using EDTA or heparin as an anticoagulant. Citrate plasma is not recommended for this assay) ? Cell culture supernatants ? Urine IL-15 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of Human IL-15 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 3 pg/ml. 100 assays.IL-15 is a recently identified novel cytokine that shares many biological properties with IL-2, including T-Cell, B-Cell and NKcell-stimulatory activities. BioVision’s Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-15. This assay employs an antibody specific for human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-15 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 β, MIP-1, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).