K4145-100? IL-6 Microplate coated with anti-rat monoclonal Ab against IL-6, 96 wells ? Lyophilized recombinant rat IL-6 standard (10 ng/vial) ? Biotinylated anti-rat IL-6 antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA, citrate) ? Cell culture supernatantsIL-6 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IL-6 in serum, plasma & cell culture supernatants. Detection Range: 62.5 pg/ml - 4000 pg/ml. 100 assays. BioVision’s rat IL-6 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for rat IL-6 coated on a 96-well plate. Standards (E. coli F25-T211) and test samples are added to the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse IL-6 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 5 pg/ml.