K4763-100? Notch-1 Microplate (Item A) coated with anti-human Notch-1, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Notch-1) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody Notch-1 (Item F), biotinylated anti-human Notch-1 ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Human Notch-1 ELISA Kit: Colorimetric Assay for Quantitative Determination of human Notch-1 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: 20 pg/ml. 100 assays. BioVision’s Human Notch-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Notch-1. This assay employs an antibody specific for human Notch-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of Notch-1 is typically less than 20 pg/ml. Detection Range: 20 pg/ml - 7000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.