K4759-100? Serum & plasma ? Cell culture media etc.Obestatin (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of Obestatin in Serum, Plasma, Cell culture supernatants etc. Detection range: 0.1-1000 pg/ml. 100 assays.BioVision’s Obestatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Obestatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Obestatin antibody, both biotinylated Obestatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Obestatin antibody. Uncompeted (bound) biotinylated Obestatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Obestatin peptide in the standard or samples. This is due to the competitive binding to Obestatin antibody between biotinylated Obestatin peptide and peptides in standard or samples. A standard curve of known concentration of Obestatin peptide can be established and the concentration of Obestatin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Obestatin is 0.1 pg/ml. The detection range for the kit is 0.1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%.