K4734-100? 1 plate coated with mouse progranulin Antibody (12 x 8-well strips) ? 1 bottle Wash Buffer 10X (50 ml) ? 1 bottle Diluent 5X (50 ml) ? 1 bottle Detection Antibody (12 ml) ? 1 vial Detector 100X (HRP Labeled Streptavidin) (150 μl) ? 1 vial human progranulin Standard (lyophilized) (8 ng) ? 1 vial human progranulin QC sample (lyophilized) ? 1 bottle TMB Substrate Solution (12 ml) ? 1 bottle Stop Solution (12 ml) ? 3 plate sealers (plastic film) Serum and Cell culture supernatants.Progranulin (Mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse progranulin in Serum, Cell culture supernatants etc. Detection Range: 0.125 ng/ml – 8 ng/ml. 100 assays.BioVision’s Progranulin (mouse) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.