K4787-100? Beta-NGF Ab coated plate (Item A), 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Human Beta-NGF Standard (Item C) ? Assay Diluent (5x) (Item E) ? Biotinylated anti-human Beta-NGF Ab (Item F) ? HRP-Streptavidin Concentrate (800x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma (collect plasma using heparin as an anticoagulant. EDTA & citrate are not recommended. ? Cell culture supernatants ? Urine Beta-NGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Beta-NGF in serum, plasma, urine & cell culture supernatants. Detection Range: 14 pg/ml – 5,000 pg/ml. 100 assays. BioVision’s Human Beta-NGF (beta-nerve growth factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Beta-NGF. This assay employs an antibody specific for human Beta-NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta-NGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Beta-NGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta-NGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Beta-NGF is typically less than 14 pg/ml. Detection Range: 14 pg/ml – 5,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.