K4778-100? LIF Microplate (Item A) coated with anti-human LIF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human LIF) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody LIF (Item F), biotinylated anti-human LIF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 320x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Lif (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Lif in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 15 pg/ml - 6000 pg/ml. 100 assays. BioVision’s Human LIF (Leukocyte inhibitory factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LIF. This assay employs an antibody specific for human LIF coated on a 96-well plate. Standards and samples are pipetted into the wells and LIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human LIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of LIF is typically less than 15 pg/ml. Detection Range: 15 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.