K4754-100? Serum & plasma ? Cell culture supernatants ? Urine TACE (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human TACE in Serum, Plasma, urine & Cell culture supernatants. Detection Limit: less than 70 pg/ml. 100 assays.BioVision’s Human TACE (TNF-alpha converting enzyme) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TACE. This assay employs an antibody specific for human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TACE is typically less than 70 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.