biovision K101-400 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-FITC Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis within 10 min. Can also differentiate Apoptosis vs Necrosis. Detection: Flow Cytometry or Fluorescence Microscopy Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.