biovision K193-100 Live cells ? Detection method- Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25 and 100 assays) ? Applications- Detection of activated caspase-3 in living cells. CaspGLOWTM Red Active Caspase-3 Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspase-3 in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader. Kit Size: 25 and 100 Assays. Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase-3 Staining Kit provides a convenient means for detecting activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.