biovision K198-25 Live cells ? Detection method- Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25 and 100 assays) ? Applications- Detection of activated caspase-8 in living cells. CaspGLOWTM Red Active Caspase-8 Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspase-8 in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader. Kit Size: 25 and 100 Assays. Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase-8 Staining Kit provides a convenient means for detecting activated caspase-8 in living cells. The assay utilizes a caspase-8 inhibitor IETD-FMK conjugated to sulfo-rhodamine (Red-IETD-FMK) as the fluorescent marker. Red-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.