biovision K200-25 Live cells ? Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm). ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25 and 100 assays) ? Applications- Detection of activated caspases in living cells. CaspSCREENTM Flow Cytometric Apoptosis Detection Kit: Simple & Convenient Kit for Detecting Activation of Caspases by Flow Cytometry in Living Cells. Kit Size: 25 and 100 Assays. Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D?R), a reported substrate for members of caspase family proteases. The caspase substrate D?R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.