PARP (Cleaved) Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot, Immunohistochemistry Western blot analysis (0.5-4 μg/ml) and immunocytochemistry (10-20 μg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.