TLR8 (TAntibody (Clone 44B05) Host:Mouse Antibody Type:Monoclonal Species Reactivity:Human Application:Western blot, Flow cytometry Western blotting (1:200-1000 dilutions) and FACS analysis. Ramos cell lysate can be used as a positive control and a 120 kDa band is detected in Western blotting. For FACS analysis, we recommend 1-4 ml per million cells. However, the optimal conditions should be determined individually.