BTF Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, mouse Application:Western blot, Immunoprecipitation Western blotting (1:500-1:2000) and immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control for Western blotting. The antibody recognizes the BTF of human and mouse origins. Reactivity to other species has not been tested.