MCAF Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, mouse Application:Western blot Western blotting (1:500 – 1:2000). HeLa cell nuclear extract (Cat.# 1641-1) can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MCAF of human and mouse origins. Reactivity to other species has not been tested.