GM-CSF Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Mouse Application:Western blot, ELISA, Neutralization 1) WB: Use 0.1-0.2 μg/ml. The detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 μg/ml of this antibody is required