利用SBI的Exo-Fect™ siRNA/miRNA转染试剂盒高效将siRNA和miRNA可靠且可重复地加载到细胞外小泡中。

1.Efficient delivery—siRNA/miRNA-loaded EVs deliver cargo to as many as 95% of target cells*

2.Validated efficacy—siRNA-loaded EVs lead to significant knockdown (>80%) of target gene expression in transfected cells**

3.No toxicity—unlike commonly-used lipid-based transfection reagents, the Exo-Fect siRNA/miRNA Transfection Reagent causes no toxicity in tested cells

4.Fast, easy workflow—go from prepared small RNA to ready-to-use, RNA-loaded EVs in less than 1.5-hours and with only 15-minutes of hands-on time

5.Minimal background—a clean-up step ensures complete removal of free small RNA and excess transfection reagent for clean, ready-to-use, RNA-loaded EVs

转染步骤：

参考文献：

1.Chen Z, et al. Therapeutic Potential of Mesenchymal Cell-Derived miRNA-150-5p-Expressing Exosomes in Rheumatoid Arthritis Mediated by the Modulation of MMP14 and VEGF. J Immunol. 2018 Oct 15;201(8):2472-2482. PMCID: PMC6176104.

2.Pegtel DM, et al. Functional delivery of viral miRNAs via exosomes. Proc Natl Acad Sci U S A. 2010;107(14):6328-33. PMCID: PMC2851954.

3.Alvarez-Erviti, L et al. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011 Apr; 29:341–345. PMID: 21423189.