biovisionK435-40KinaseSTAR Akt Activity Assay Kit, Akt, active kinase, active kinases, Akt, kinase activity assay, protein kinase, Akt assay, Akt assay kit, akt? Detection method- Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions ? Species reactivity- Mammalian ? Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Cell and tissue lysates